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KMID : 1025520000420050591
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Journal of Animal Science and Technology
2000 Volume.42 No. 5 p.591 ~ p.604
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Separation Method for Anaerobic Microbial Fractions(Bacteria , Protozoa and Fungi) from Rumen Fluids
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신기준/Shin, K. J.
이성실/하종규/박정근/케이제이쳉/Lee, S. S./Ha, J. K./Park, J. G./Cheng, K. J.
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Abstract
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Separation methods for rumen microbial fractions contained the bacterial, protozoal and fungal fraction which are the most useful to assess the relative contribution of microbial groups for digestion of feedstuffs and the useful tools to study the rumen microbiology. The fractionation methods have been classified into three main categories; physical method based on cell size and weight, chemical method based on the microbial sensitivity to some chemicals, and physicochemical methods. After microbial groups were selected by physical treatment(centrifuge, filtering and autoclaves), chemical treatment(by the addition of antibiotics and/or some chemicals) and physicochemical treatments of rumen fluid, enumerated each microbial fraction using microscopy and roll-tube methods, and then estimated each separation method. This study emphasized the relative advantageous of the different methods and the factors to be kept in mind when choosing the most appropriate method for a definite goal.
Physical treatment(filteration with nylon cloth had larger pore size about 500㎛ or centrifugation) was estimated potentially sufficient to separate protozoal fraction alone from the rumen fluids, due to the phenomenal differences their cell size and weight compared to other microbes, but must be considered of the some kinds of bacteria and/or fungal spores can be survive in protozoa) cell. When separating the microbial groups from the rumen fluids, a physicochemical method is probably the best method, especially when needed separation of bacterial fraction and fungal fraction. And the physicochemical method gave more precise results are presented in Fig. 4 in the main segments of the paper.
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